Paper We're Discussing This Week:

Walker EY, Pohl S, Denison RN, Barack DL, Lee J, Block N, Ma WJ, Meyniel F. (2023) Studying the neural representations of uncertainty. Nature Neuroscience, 10.1038/s41593-023-01444-y

Previously:

Asokan M.M., Watanabe Y., Kimchi E.Y., Polley D.B. (2023) Potentiation of cholinergic and corticofugal inputs to the lateral amygdala in threat learning. Cell Reports 42: 113167.

Yu H, Chen L, Lei H, Pi G, Xiong R, Jiang T, Wu D, Sun F, Gao Y, Li Y, Peng W, Huang B, Song G, Wang X, Lv J, Jin Z, Ke D, Yang Y, Wang JZ. (2022) Infralimbic medial prefrontal cortex signalling to calbindin 1 positive neurons in posterior basolateral amygdala suppresses anxiety- and depression-like behaviours. Nat Commun. 13:5462.

Cummings KA, Bayshtok S, Dong TN, Kenny PJ, Clem RL. (2022) Control of fear by discrete prefrontal GABAergic populations encoding valence-specific information. Neuron, doi: 10.1016/j.neuron.2022.07.004.

Yanpallewar S, Tomassoni-Ardori F, Palko ME, Hong Z, Kiris E, Becker J, Fulgenzi G, Tessarollo L. (2022) TrkA-cholinergic signaling modulates fear encoding and extinction learning in PTSD-like behavior. Transl Psychiatry 12(1):111.

Ben-Ami Bartal I, Rodgers DA, Bernardez Sarria MS, Decety J, Mason P. (2014) Pro-social behavior in rats is modulated by social experience. Elife 3:e01385

Huggins A.A, Weis C.N., Parisi E.A., Bennett K.P., Miskovic V., Larson C.L. (2021) Neural substrates of human fear generalization: A 7T-fMRI investigation. Neuroimage 239: 118308.

Fu X, Teboul E, Weiss GL, Antonoudiou P, Borkar CD, Fadok JP, Maguire J, Tasker JG. (2022) Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice. Nat Comm 13: 1290.

LA Osso, KA Rankin, JR Chan (2021) Experience-dependent myelination following stress is mediated by the neuropeptide dynorphin Neuron 109: 3619-3632.e5

Rachel A. Walker, Rebecca L. Suthard, Taylor N. Perison, Nora M. Sheehan, Caitlin C. Dwyer, Jillian K. Lee, Eghosa K. Enabulele, Madelyn H. Ray and Michael A. McDannald (2022) Dorsal raphe 5-HT neurons utilize, but do not generate, negative aversive prediction errors eNeuro. 0132-21.2022

Joffe ME, Maksymetz J, Luschinger JR, Dogra S, Ferranti AS, Luessen DJ, Gallinger IM, Xiang Z, Branthwaite H, Melugin PR, Williford KM, Centanni SW, Shields BC, Lindsley CW, Calipari ES, Siciliano CA, Niswender CM, Tadross MR, Winder DG, Conn PJ. (2022) Acute restraint stress redirects prefrontal cortex circuit function through mGlu 5 receptor plasticity on somatostatin-expressing interneurons. Neuron S0896-6273: 01044-8.

Sánchez-Bellot C, AlSubaie R, Mishchanchuk K, Wee RWS, MacAskill AF. (2022) Two opposing hippocampus to prefrontal cortex pathways for the control of approach and avoidance behaviour. Nat Comm 13: 339

Whittle N, Fadok J, MacPherson KP, Nguyen R, Botta P, Wolff SBE, Müller C, Herry C, Tovote P, Holmes A, Singewald N, Lüthi A, Ciocchi S. (2021) Central amygdala micro-circuits mediate fear extinction. Nat Comm 12: 4156

Lazic SE. (2010) The problem of pseudoreplication in neuroscientific studies: is it affecting your analysis? BMC Neurosci 11:5.

Yu W, Pati D, Pina MM, Schmidt KT, Boyt KM, Hunker AC, Zweifel LS, McElligott ZA, Kash TL (2021) Periaqueductal gray/dorsal raphe dopamine neurons contribute to sex differences in pain-related behaviors. Neuron 109:1365-1380.

Benamer N, Vidal M, Balia M, Angulo MC. (2020) Myelination of parvalbumin interneurons shapes the function of cortical sensory inhibitory circuits. Nat Communications 11:5151.

Cai LX, Pizano K, Gundersen GW, Hayes CL, Fleming WT, Holt S, Cox JM, Witten IB. (2020) Distinct signals in medial and lateral VTA dopamine neurons modulate fear extinction at different times. eLife: 9:e54936. doi:10.7554/eLife.54936.

Shine JM, Müller EJ, Munn B, Cabral J, Moran RJ, Breakspear M. (2021)
Computational models link cellular mechanisms of neuromodulation to large-scale neural dynamics. Nat Neurosci. doi: 10.1038/s41593-021-00824-6.

Khalighinejad N, Bongioanni A, Verhagen L, Folloni D, Attali D, Aubry JF, Sallet J, Rushworth MFS. (2020) A Basal Forebrain-Cingulate Circuit in Macaques Decides It Is Time to Act. Neuron 105: 37-384.

Sohn JMB, de Souza STF, Raymundi AM, Bonato J, de Oliveira RMW, Prickaerts J, Stern CA. (2020) Persistence of the extinction of fear memory requires late-phase cAMP/PKA signaling in the infralimbic cortex. Neurobiol Learn Mem. 172:107244.

Kim CK, Sanchez MI, Hoerbelt P., Fenno LE, Malenka RC, Deisseroth K, Ting AY (2020) A Molecular Calcium Integrator Reveals a Striatal Cell Type Driving Aversion. Cell 183: 2003-2019.

Pan S., Mayoral S.R., Choi H.S., Chan J.R., Kheirbek M. (2020) Preservation of a remote fear memory requires new myelin formation. Nat. Neurosci 23: 487-499.

Qin C, Bian XL, Wu HY, Xian JY, Cai CY, Lin YH, Zhou Y, Kou XL, Chang L, Luo CX, Zhu DY. (2020) Dorsal Hippocampus to Infralimbic Cortex Circuit is Essential for the Recall of Extinction Memory. Cereb Cortex Nov 14;bhaa320.

Cho KKA, Davidson TJ, Bouvier G, Marshall JD, Schnitzer MJ, Sohal VS. (2020) Cross-hemispheric gamma synchrony between prefrontal parvalbumin interneurons supports behavioral adaptation during rule shift learning. Nat Neurosci. 23:892-902.

Companion discussion paper:

Buzsáki G, Schomburg EW. (2015) What does gamma coherence tell us about inter-regional neural communication? Nat Neurosci.18:484-9.

Lovett-Barron M, Chen R, Bradbury S, Andalman AS, Wagle M, Guo S, Deisseroth K. (2020) Multiple convergent hypothalamus-brainstem circuits drive defensive behavior. Nat Neurosci.

McMurray KMJ, Gray A, Horn P, Sah R. (2020) High Behavioral Sensitivity to Carbon Dioxide Associates with Enhanced Fear Memory and Altered Forebrain Neuronal Activation. Neuroscience 429: 92-105.

Day HLL, Suwansawang S, Halliday DM, Stevenson CW. (2020) Sex differences in auditory fear discrimination are associated with altered medial prefrontal cortex function. Sci Rep. 10:6300.

van Holstein M., MacLeod P.E., Floresco S. (2019) Basolateral amygdala - nucleus accumbens circuitry regulates optimal cue-guided risk/reward decision making. Prog Neuropsychopharm and Biol Psych. 98: 109830.

Dolensek N, Gehrlach DA, Klein AS, Gogolla N. (2020) Facial expressions of emotion states and their neuronal correlates in mice. Science 368 :89-94.

Dabney W., Kurth-Nelson Z., Uchida N., Starkweather C.K., Hassabis D., Munos R., Botvinick M. (2020) A Distributional Code for Value in Dopamine-Based Reinforcement Learning. Nature 577: 671-675.

Gao C, Leng Y, Ma J, Rooke V, Rodriguez-Gonzalez S, Ramakrishnan C, Deisseroth K, Penzo MA. (2020) Two genetically, anatomically and functionally distinct cell types segregate across anteroposterior axis of paraventricular thalamus. Nat Neurosci. 23:217-228.

Zhang B, Ma S, Rachmin I, He M, Baral P, Choi S, Gonçalves WA, Shwartz Y, Fast EM, Su Y, Zon LI, Regev A, Buenrostro JD, Cunha TM, Chiu IM, Fisher DE, Hsu YC. (2020) Hyperactivation of sympathetic nerves drives depletion of melanocyte stem cells. Nature 577:676-681.

Scheggia D, Managò F, Maltese F, Bruni S, Nigro M, Dautan D, Latuske P, Contarini G, Gomez-Gonzalo M, Requie LM, Ferretti V, Castellani G, Mauro D, Bonavia A, Carmignoto G, Yizhar O, Papaleo F. Somatostatin interneurons in the prefrontal cortex control affective state discrimination in mice (2020) Nature Neurosci 23:47-60.

Blume R.S., Padival M., Urban J.H., Rosenkranz A.J. (2019) Disruptive effects of repeated stress on basolateral amygdala neurons and fear behavior across the estrous cycle. Sci Rep 9:12292. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707149/

Forkosh O, Karamihalev S, Roeh S, Alon U, Anpilov S, Touma C, Nussbaumer M, Flachskamm C, Kaplick PM, Shemesh Y, Chen A.(2019) Identity domains capture individual differences from across the behavioral repertoire. Nature Neuroscience. Nov. 4.

Kasugai Y, Vogel E, Hörtnagl H, Schönherr S, Paradiso E, Hauschild M, Göbel G, Milenkovic I, Peterschmitt Y, Tasan R, Sperk G, Shigemoto R, Sieghart W, Singewald N, Lüthi A, Ferraguti F. (2019) Structural and Functional Remodeling of Amygdala GABAergic Synapses in Associative Fear Learning. Neuron 104:1-14.

Smith EH, Horga G, Yates MJ, Mikell CB, Banks GP, Pathak YJ, Schevon CA, McKhann II GM, Hayden BY, Botvinick MM, Sheth SA. (2019) Widespread temporal coding of cognitive control in the human prefrontal cortex. Nature Neuro, https://www.nature.com/articles/s41593-019-0494-0

Du. J., Price M.P., Taugher R.J., Gigsby D., Ash J.J., Stark A.C., Saad M.Z.H., Singh K., Mandal J., Wemmie J.A., Welsh M.J. (2017) Transient acidosis while retrieving a fear-related memory enhances its lability. eLife;6:e22564.

Hasan MT, Althammer F, Silva da Gouveia M, Goyon S, Eliava M, Lefevre A, Kerspern D, Schimmer J, Raftogianni A, Wahis J, Knobloch-Bollmann HS, Tang Y, Liu X, Jain A, Chavant V, Goumon Y, Weislogel JM, Hurlemann R, Herpertz SC, Pitzer C, Darbon P, Dogbevia GK, Bertocchi I, Larkum ME, Sprengel R, Bading H, Charlet A, Grinevich V. (2019) A Fear Memory Engram and Its Plasticity in the Hypothalamic Oxytocin System. Neuron.103:133-146.e8.

Yaeger CE, Ringach DL, Trachtenberg JT. (2019) Neuromodulatory control of localized dendritic spiking in critical period cortex. Nature 567:100-104.

Stephenson-Jones M., Bravo-Rivera C., Ahrens S., Furlan A., Ferndandes-Henriques C., Li B. bioRxiv preprint, posted 3/31/19. https://www.biorxiv.org/content/10.1101/594887v1

Falkner AL, Grosenick L, Davidson TJ, Deisseroth K, Lin D. (2016) Hypothalamic control of male aggression seeking behavior. Nat Neurosci 19:596.

Zhang K., Chen CD, Monosov IE (2019) Novelty, Salience, and Surprise Timing are Signaled by Neurons in the Basal Forebrain. Current Biology 29:134.

Baek J, Lee S, Cho T, Kim SW, Kim M, Yoon Y, Kim KK, Byun J, Kim SJ, Jeong J, Shin HS. (2019) Neural Circuits Underlying a Psycho-therapeutic Regimen for Fear Disorders. Nature, 566: 339.

Pryluk R., Kfir Y., Gelbard-Sagiv H., Fried I., Paz R. (2019) A Tradeoff in the Neural Code Between Regions and Species. Cell 176: 597-609.

Popper, K. (1935) Theory. In The Logic of Scientific Discovery. London and New York: Routledge Classics.

Ronovsky M, Zambon A, Cicvaric A, Boehm V, Hoesel B, Moser BA, Yang J, Schmid JA, Haubensak WE, Monje FJ, Pollak DD. (2019) A role for miR-132 in learned safety. Sci Reports 9: 528.

Seo C, Guru A, Jin M, Ito B, Sleezer BJ, Ho YY, Wang E, Boada C, Krupa NA, Kullakanda DS, Shen CX, Warden MR. (2019) Intense threat switches dorsal raphe serotonin neurons to a paradoxical operational mode. Science. 363:538-542.

Corder G., Ahanonu B., Grewe B.F., Wang D., Schnitzer M.J., Scherrer G. (2019) An amygdalar neural ensemble that encodes the unpleasantness of pain. Science 363: 276-281.

Zhang X., Li B. (2018) Population coding of valence in basolateral amygdala. Nat Comm 9: 5195.

Nelson A., Mooney R. (2016) The basal forebrain and motor cortex provide convergent yet distinct movement-related inputs to the auditory cortex. Neuron, 90: 635-648.

Allsop SA, Wichmann R, Mills F, Burgos-Robles A, Chang CJ, Felix-Ortiz AC, Vienne A, Beyeler A, Izadmehr EM, Glober G, Cum MI, Stergiadou J, Anandalingam KK, Farris K, Namburi P, Leppla CA, Weddington JC, Nieh EH, Smith AC, Ba D, Brown EN, Tye KM. (2018) Corticoamygdala Transfer of Socially Derived Information Gates Observational Learning. Cell, 10.1016/j.cell.2018.04.004

Ren J, Friedmann D, Xiong J, Liu CD, Ferguson BR, Weerakkody T, DeLoach KE, Ran C, Pun A, Sun Y, Weissbourd B, Neve RL, Huguenard J, Horowitz MA, Luo L. (2018) Anatomically Defined and Functionally Distinct Dorsal Raphe Serotonin Sub-systems. Cell, doi: 10.1016/j.cell.2018.07.043

Aitta-Aho T, Hay YA, Phillips BU, Saksida LM, Bussey TJ, Paulsen O, Apergis-Schoute J. (2018) Basal Forebrain and Brainstem Cholinergic Neurons Differentially Impact Amygdala Circuits and Learning-Related Behavior. Curr Biol, doi: 10.1016/j.cub.2018.06.064.

Giardino WJ, Eban-Rothschild A, Christoffel DJ, Li SB, Malenka RC, de Lecea L. (2018) Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states. Nat Neurosci 21:1084-1095.

Ahrens S., Wu M.V., Furlan A., Hwang G-R., Paik R., Li H., Penzo M., Tollukuhn J., Li B. (2018) A central extended amygdala circuit that modulates anxiety. J Neurosci 38: 5567-5583.

Marek R, Xu L, Sullivan RKP, Sah P. (2018) Excitatory connections between the prelimbic and infralimbic medial prefrontal cortex show a role for the prelimbic cortex in fear extinction. Nat Neurosci 21:654-658.

Ashley J, Cordy B, Lucia D, Fradkin LG, Budnik V, Thomson T. (2018) Retrovirus-like Gag Protein Arc1 Binds RNA and Traffics across Synaptic Boutons. Cell 172: 262-274.

Marek R, Jin J, Goode TD, Giustino TF, Wang Q, Acca GM, Holehonnur R, Ploski JE, Fitzgerald PJ, Lynagh T, Lynch JW, Maren S, Sah P. (2018) Hippocampus-driven feed-forward inhibition of the prefrontal cortex mediates relapse of extinguished fear. Nat Neuroscie 21:384-392.

Salay N.D.S., Ishiko N., Huberman A.D. (2018) A midline thalamic circuit determines reactions to visual threat. Nature, doi: 10.1038/s41586-018-0078-2.

Kuchibhotla KV, Gill JV, Lindsay GW, Papadoyannis ES, Field RE, Sten TA, Miller KD, Froemke RC (2017) Parallel processing by cortical inhibition enables context-dependent behavior. Nat Neurosci 20: 62-71.

Madarasz TJ, Diaz-Mataix L, Akhand O, Ycu EA, LeDoux JE, Johansen JP. (2016) Evaluation of ambiguous associations in the amygdala by learning the structure of the environment. Nat Neurosci 19: 965

Steinmetz NA, Buetfering C, Lecoq J, Lee CR, Peters AJ, Jacobs EAK, Coen P, Ollerenshaw DR, Valley MT, de Vries SEJ, Garrett M, Zhuang J, Groblewski PA, Manavi S, Miles J, White C, Lee E, Griffin F, Larkin JD, Roll K, Cross S, Nguyen TV, Larsen R, Pendergraft J, Daigle T, Tasic B, Thompson CL, Waters J, Olsen S, Margolis DJ, Zeng H, Hausser M, Carandini M, Harris KD. (2017) Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines. eNeuro. 4(5). pii: ENEURO.0207-17.2017.

Murugan M, Jang HJ, Park M, Miller EM, Cox J, Taliaferro JP, Parker NF, Bhave V, Hur H, Liang Y, Nectow AR, Pillow JW, Witten IB.(2017) Combined social and spatial coding in a descending projection from the prefrontal cortex. Cell 171: 1663 - 1677.

Karl Popper, The Logic of Scientific Discovery, Routledge Press, 1959 (Chapters I and II).

Espinosa N, Alonso A, Morales C, Espinosa P, Chávez AE, Fuentealba P. (2017) Basal Forebrain Gating by Somatostatin Neurons Drives Prefrontal Cortical Activity. doi.org/10.1093/cercor/bhx302

Davis P., Zaki Y., Maguire J., Reijmers LG. (2017) Cellular and oscillatory substrates of fear extinction learning.Nat. Neurosci: doi: 10.1038/nn.4651

Martin-Fernandez M., Jamison S., Robin L.M., Zhao Z., Martin E.D., Aguilar J., Benneyworth M.A., Marsicano G., Araque A. (2017) Synapse-specific astrocyte gating of amygdala-related behavior. Nat Neurosci: doi:10.1038/nn.4649.

Wimmer R.D., Schmitt L.I., Davidson T.J., Nakajima M., Deisseroth K., Halassa M.M. (2015) Thalamic control of sensory selection in divided attention. Nature 526: 705-709.

Han W., Tellez L.A., Rangel Jr. M.J., Motta S.C., Zhang X., Perez I.O., Canteras N.S., Shammah-Lagnado S.J., van den Pol A.N., de Araujo I.E. (2017) Integrated control of predatory hunting by the central nucleus of the amygdala. Cell 168:311-324.

Gielow M.R., Zaborzsky L. (2017) The input-output relationship of the cholinergic basal forebrain. Cell Rep 18:1870-1830.

Halladay L.R., Blair H.T. (2015) Distinct ensembles of medial prefrontal cortex neurons are activated by threatening stimuli that elicit excitation vs. inhibition of movement. J Neurophysiol 114:793-807.

Grewe B.F., Gründemann J., Kitch L.J., Lecoq J.A., Parker J.G., Marshall J.D., Larkin M.C., Jercog P.E., Grenier F., Li J.Z., Lüthi A., Schnitzer M.J. (2017) Neural ensemble dynamics underlying a long-term associative memory. Nature 543: 670-675.

Kim W.B., Cho J-H. (2017) Synaptic targeting of double-projecting ventral CA1 hippocampal neurons to the medial prefrontal cortex and basal amygdala. J Neurosci 37:4868-4882.

Otis J.M., Namboodiri V.M., Matan A.M., Voets E.S., Mohorn E.P., Kosyk O., McHenry J.A., Robinson J.E., Resendez S.L., Rossi M.A., Stuber G.D. (2017) Prefrontal cortex output circuits guide reward seeking through divergent cue encoding. Nature 543: 103-107.

Wallis C.U., Cardinal R.N., Alexander L., Roberts A.C., Clarke H.F. (2017) Opposing roles of primate areas 25 and 32 and their putative rodent homologs in the regulation of negative emotion. PNAS 114: E4075-4084.

Gruene T.M., Lipps J., Rey C.D., Bouck A., Shansky R.M. (2014) Heat exposure in female rats elicits abnormal fear expression and cellular changes in prefrontal cortex and hippocampus. Neurobiol Learn Mem 115: 38-42.

Miniscope workshop (Becky).

Day H.L.L., Reed M.M., Stevenson C.W. (2016) Sex differences in discriminating between cues predicting threat and safety. Neurobiol Learn Mem 133: 196-203.

Danielle and Margo are presenting posters on April 5 & 6 this week at the Undergraduate Research Conference.

A competitive inhibitory circuit for selection of active and passive fear responses.

Fadok JP, Krabbe S, Markovic M, Courtin J, Xu C, Massi L, Botta P, Bylund K, Müller C, Kovacevic A, Tovote P, Lüthi A.(2017) Nature, 542: 196.

An inhibitory pull-push circuit in frontal cortex.

Garcia-Junco-Clemente P., Ikrar T., Tring E., Xu X., Ringach D.L., Trachtenberg J.T. Nat Neurosci 20:389.

Gq/5-HT2c receptor signals activate a local GABAergic inhibitory feedback circuit to modulate serotonergic firing and anxiety in mice.

Spoida K, Masseck OA, Deneris ES, Herlitze S. PNAS 111:6479.

An intra-amygdala circuit specifically regulates social fear learning

Twinning R.C., Vantrease J.E., Love S., Padival M., Rosenkranz J.A. Nat Neurosci 20: 459. (2017)

Distinct hippocampal pathways mediate dissociable roles of context and memory retrieval.

Xu C., Krabbe S., Grundemann J., Botta P., Fadok J., Osakada F., Saur D., Grewe B.F., Schnitzer M.J., Callaway E.M., Luthi A.

Basal forebrain neuronal inhibition enables rapid behavioral stopping

Jeffrey D. Mayse, Geoffrey M. Nelson, Irene Avila, Michela Gallagher, and Shih-Chieh Lin,

Dysregulation of Prefrontal Cortex-Mediated Slow-Evolving Limbic Dynamics Drives Stress-Induced Emotional Pathology.

Hultman R, Mague SD, Li Q, Katz BM, Michel N, Lin L, Wang J, David LK, Blount C, Chandy R, Carlson D, Ulrich K, Carin L, Dunson D, Kumar S, Deisseroth K, Moore SD, Dzirasa K.

Cortical cholinergic signaling controls the detection of cues

Howard J. Grittona , William M. Howea , Caitlin S. Mallorya , Vaughn L. Hetricka , Joshua D. Berkea , and Martin Sartera,1

A shared neural ensemble links distinct contextual memories encoded close in time

Denise J. Cai1 *, Daniel Aharoni1,2,3*, Tristan Shuman2,3*, Justin Shobe1 *, Jeremy Biane4,5, Weilin Song1 , Brandon Wei1 , Michael Veshkini1 , Mimi La-Vu1 , Jerry Lou2,3, Sergio E. Flores2,3, Isaac Kim1 , Yoshitake Sano1 , Miou Zhou1 , Karsten Baumgaertel6, Ayal Lavi1 , Masakazu Kamata7 , Mark Tuszynski4,5, Mark Mayford6, Peyman Golshani2,3 & Alcino J. Silva1

Congratulations to Dr. Carolina M. Fernandes-Henriques!!

March 14, 2024

On January 16th, Carolina beautifully defended her thesis, “The Role of the Infralimbic-Basal Forebrain Pathway in Fear Extinction”. It was a wonderful event and the first manuscript from her work is on its way out.

A heartfelt thank you to Carolina’s fantastic committee: Rob Froemke, Allyson Friedman, Andy Delamater, and Susana Mingote. Their contributions and discussions were most welcome and appreciated.

As Carolina is getting a PhD, she showed us her matrilineal lineage in Portugal. Each succeeding generation achieves a more advanced degree.

Lots of love came in from the room and via Zoom from Portugal.

Dr. Henriques’s celebratory cake and cheese bounty

Congratulations to Rhonda and Emma!

December 06, 2023

Congratulations to Rhonda (seen here as Green Fluorescent Protein) on passing her qualifying exam and moving on to candidacy!

Congratulations to Emma (seen here as Dylight 405) for getting accepted to the Advanced Neuroimaging Techniques course at Max Planck and for winning a Travel Award!

The Fluorescent Proteins at the Biology Halloween party

SFN 2023

December 06, 2023

We had a great time catching up with all the people, the science, and the tech at SfN in DC.

Breakfast Club style at SfN in DC (missing Nawshin, George, and Katie)

Carolina showing her thesis work on circuit communication during extinction learning

Emma showing her thesis work on oligodendrocyte - neuron interactions in learning

The mysteries of the Olive Garden were sampled en route back to NYC

Catch up with us at SFN

November 11, 2023

We will be at SFN, presenting our work and that of others.

Webinar on Making Posters for SFN

November 01, 2023

If you’re new to making posters, take a look at our SFN Webinar on “How To Make and Present a Poster”. In it, Marina Picciotto, Steve Maren, and I discuss various aspects of making a poster (from visuals to content), how to present to different audiences, and how to organize post-presentation networking.

https://neuronline.sfn.org/professional-development/how-to-make-and-present-a-poster-for-neuroscience-2023

Also, take a look at the webinar “Maximizing Your Time at SFN’s Annual Meeting” with Janice Naegele and Veronica Galvin, for strategies on how to prevent feeling overwhelmed.

Why am I scared of bridges?

October 06, 2023

BBC Crowd Science (produced by Hannah Fisher, hosted by Caroline Steel) covered phobias in an episode titled “Why am I scared of bridges?”, where we discussed the circuitry of fear, and Caroline covers lots of other aspects of phobias, such as cultural influences and therapeutic approaches. Caroline even gets therapy for her phobia of elevators. Take a listen.

Pavlovian 2023 in Austin TX

September 28, 2023

We had a wonderful time presenting our work and learning from others at the Pavlovian conference in Austin. Lots of interesting talks, posters, and people.

Carolina and Emma showing their projects

Emma’s confocal images of PV cells are great for staging a talk that showcases the role of prefrontal interneurons

Jinah and Mia went full TX with the bolo ties

IBRO 2023

September 14, 2023

The IBRO conference in Granada was lots of fun with 5 days of great science in a beautiful city. Sara Mederos (Wellcome Center, UCL) and Mario Martin-Fernandez (University of Bordeaux) organized a wonderful symposium, “From Negative Emotion to Defensive Behaviors”, where Sara, Mario, Rony Paz (Weizmann Institute), and I shared new data on circuits of emotion regulation. Olé!

Lab life catch up!

August 11, 2023

A lot has happened - it’s time to turn on the time machine and catch up a little bit.

Our summer student Ariana did a wonderful job presenting her summer project at the NYU GSTEM event to wrap up her 5 weeks with us in the lab. Special thanks to George and Katie for teaching Ariana about behavioral analysis and cell sorting, and to Mia for showing her all sorts of lab techniques!

Carolina won the Best Scientific Presentation Award at the CUNY Sci Comm event held at the Advanced Science Research Center.

Society of Biological Psychiatry - Symposium on The Role of Somatostatin Interneurons in Affective and Cognitive Disorders with Nikki Crowley (Penn State), Thomas Prevot (University of Toronto), and Dave Kupferschmidt (NIMH)

Yuval presented her work at the Eastern Psychological Association in Boston and at the Hunter UGRC, and Nawshin showed her work at the CUNY wide STEM conference and at the Hunter UGRC

Secret Lab Mate Dinner of 2022 was actually celebrated in 2023!

How could we not attend the “Life of a Neuron” exhibit at Artechouse?

SFN 2022 in San Diego was little photographed, but much enjoyed

CUNY Neuro Seminar Fri 2/10: Alain Frigon

February 10, 2023

“Inhibition and facilitation of spinal circuits by sensory inputs from the lumbar and perineal regions after spinal cord injury”

Dr. Alain Frigon (Université de Sherbrooke, Canada)

Email: el1417 at hunter dot cuny dot edu for link to join seminar

CUNY Neuro Seminar: Orie Shafer - Jan 27 @3pm EST!

January 27, 2023

Kicking off our Spring 2023 Seminar Series is our own Orie Shafer!

Dr. Orie Shafer (ASRC, CUNY)

Circadian and Homeostatic Regulation of Sleep in an Insect Brain

Sleep is the foundation of health and well-being and is regulated by two major processes residing within the animal brain: circadian timekeeping and homeostatic sleep control. These processes are highly conserved and genetically tractable invertebrate model systems have consistently enriched and informed our understanding their operation in mammalian brains. In this seminar, I will briefly summarize ~20 years of my lab's work on the circadian clock in Drosophila brain and highlight new work focused on how circadian timekeeping and sleep homeostasis cooperate to control sleep timing and duration in the fly.

To join, email: el1417 at hunter dot cuny dot edu

Dec 5 Biology Seminar: Dr. Leora Yetnikoff

December 04, 2022

Monday, December 5th at 12:30pm EST

“Axo-glial interactions between midbrain dopamine axons and oligodendrocyte lineage cells and their implications for experience-dependent myelination.”

Dr. Leora Yetnikoff (CSI, CUNY)

email el1417 at hunter dot cuny dot edu for a link to join

Our new myelin work made a Pavlovian debut

October 03, 2022

What a terrific conference! We very much enjoyed catching up with all the interesting new projects and directions in the field of learning, Emma showed our new work looking at experience-dependent myelin remodeling in the prefrontal cortex, we ran a lively symposium on the Generalization of Learning, attended breakout sessions and enjoyed the Women In Science luncheon to boot. Looking forward to next year in Austin!

The Pavlovian Conference is coming!

September 28, 2022

Nesha Burghardt and I will be chairing a symposium on The Generalization of Learning this coming Saturday at the Pavlovian conference in Milwaukee. We are looking forward to all the interesting ways the speakers think about generalization in their work:

Jelena Radulovic: “Retrieval-based generalization of aversive conditioning”

Larry Zweifel: “The role of dopamine in threat generalization”

Maria Geffen: “A cortico-thalamic circuit for learning generalization”

Joey Dunsmoor: “Latent associative structures facilitate higher-order transfer of learned fear”

The conference is packed with wonderful talks and symposia on the interactions between stress and learning, social and appetitive learning, habit formation, safety learning, effects of drugs and alcohol on learning and memory, learning about context, and on!

Summer 2022 is over?

September 14, 2022

The summer flew by with lots of activity and little posting about it. One highlight was going to FENS, where Carolina showed our work on the medial prefrontal cortex-basal forebrain circuit in extinction. It was a great meeting, seeing colleagues in person was a treat, and Paris is still beautiful!

Looks like there are some well-clustered single units around the Eiffel Tower.

New Paper Out!

May 06, 2022

Our new paper, Prelimbic cortex drives discrimination of non-aversion via amygdala somatostatin interneurons is now out online at Neuron. This paper grew out of our interest in the mechanisms the brain uses to discriminate non-threatening stimuli, and suppress fear. Our findings are summarized in this graphic, with a deeper dive into the data below.

Inputs from the medial prefrontal cortex (mPFC) to the amygdala drive activation of inhibitory somatostatin-expressing interneurons (SOM). This desynchronizes amygdala pyramidal cells, decreasing defensive freezing and generalized fear (left). When the mPFC input is absent, this mechanism fails, and more generalized fear is observed.

We first show that when non-threatening stimuli are remembered and fear is suppressed, the inhibitory somatostatin-expressing (SOM) interneurons of the basolateral amygdala become active.

2-photon recordings of somatostatin (SOM) -expressing interneurons in the basolateral amygdala show increased activation to the non-threatening CS- (shown in blue) relative to the threatening CS+ (shown in red).

When just the non-threatening CS- is remembered, there is more activity in SOM interneurons than in parvalbumin-expressing (PV) interneurons of the amygdala.

Interestingly, when we inhibited amygdala SOM interneurons, this resulted in increased fear generalization. The potential cellular mechanism of this behavioral finding is intriguing because SOM interneurons target dendrites of amygdala pyramidal cells, and could act to filter out incoming input, preventing the pyramidal cells from synchronous activation. And indeed, our recordings show that during a non-threatening cue, amygdala activity is less synchronous, but when SOM interneurons are inhibited, it becomes more synchronized in the theta-band response.

During the non-threatening CS-, amygdala activity shows less theta reset than during the aversive CS+ (left). However, when SOM interneurons are inhibited, CS- theta reset increases, indicating more synchronous activation of amygdala cell firing (right).

When SOM interneurons are inhibited in the amygdala during the non-threatening CS- (light blue line), the firing rate of pyramidal cells becomes more modulated by the theta-frequency.

Given that the prelimbic cortex is important for fear discrimination learning, we inhibited it, to see if it affects SOM activity and synchrony in the amygdala, as well as behavior. We found that inhibiting the prelimbic cortex increased generalized fear, decreased SOM interneuron activity in the amygdala, and resulted in increased cue-evoked theta synchrony. We also find, that stimulating prelimbic inputs to the amygdala during memory retrieval, improved discrimination in animals that otherwise showed generalized fear.

SOM cells in the amygdala are active during the CS- when the prelimbic cortex of the mPFC is active (left), but are not active during the CS- when the PL is inhibited (right).

Optogenetic stimulation of PL inputs to the amygdala during retrieval of aversive and non-threatening cues decreases defensive behavior during the non-threatening cue, which results in less generalized fear.

There were many people involved in this work, but I’d like to highlight the following folks:

Friday 5/6 Seminar: Noa Ofen, Ph.D.

May 05, 2022

“The development of memory systems in the human brain”

Dr. Noa Ofen, PhD (Wayne State University)

3:00pm EST - online seminar

please email el1417 at hunter.cuny.edu for link

CUNY Neuro Seminar: Fri, April 29 @ 3pm EST

April 29, 2022

Environmental contributions to Alzheimer's disease

Dr.Masashi Kitazawa, Ph.D. (University of California, Irvine)

This is an online seminar.

Please email el1417 at hunter dot cuny dot edu for a link to attend

Monday 4/11 Biology Seminar: Madeline Shea

April 11, 2022

Calmodulin Interactions with Intracellular Fibroblast Growth Factors and Voltage-Gated Sodium Channels

Madeline Shea, Ph.D. (University of Iowa)

12pm EST

online seminar, email el1417 at hunter dot cuny dot edu for link to attend

Friday (4/8) CUNY Neuro seminar: Alex Wade

April 08, 2022

Functional neuroimaging of human visual pathways underlying the perception of motion in depth.

Alex Wade, Ph.D. (University of York, UK)

In the middle of the 20th century it was noted that the mathematical operations required to compute motion in depth (MID) from binocular inputs can be executed in two different sequences. One sequence computes the rate of change of retinal disparity ('changing disparity', CD). The other sequence computes the disparity of two monocular velocity signals ('inter-ocular velocity difference, IOVD). In this talk, I present recent work from my lab examining the functional specializations of these two pathways as well as their computational time courses and anatomical substrates.

3pm EST - Seminar Online

Email for link: el1417 at hunter dot cuny dot edu